波浪的非线性频散关系   总被引：1，自引：0，他引：1       下载免费PDF全文

张扬  李瑞杰  郑金海 《水科学进展》2004,15(4):448-453

在概括和总结现有非线性频散关系的基础上,给出了非线性频散关系的通式形式及其显式表达式。通过与原频散关系的比较可知显式形式具有很高的精度,与隐式形式吻合很好。利用频散关系的显式形式,结合含非线性项的缓坡方程,得到考虑非线性弥散影响的波浪变形模型。模型中对采用非线性频散关系和修正的非线性频散关系的计算结果进行了比较。结果表明,用新的频散关系的显式表达式得到的模型更加精确、合理。  相似文献   

紧凑二叉树表示法──一种有效的图像表达技术     

谈国新 《国土资源遥感》1995,6(3):47-54

本文介绍了一种新的图像表达技术──紧凑二叉树表示法,给出了该技术的编码方法,讨论了基于紧凑二叉树表示的图像操作过程。通过与其它图像表达技术的比较和分析表明,紧凑二叉树表示具有较高的时空效率。  相似文献   

在CAD使用中加入AUTOLSIP编程的研究     

程学忠 《物探化探计算技术》2001,23(2):166-170

作者在文中介绍了为适应新形势，在计算机辅助设计AUTOCAD使用中，灵活运用AUTOLISP语言编制程序绘制图形，应将自学重点放到掌握LISP语言的语法规则，用一个典型的自学内容清单，说明在自学中应增加的学习内容，列举了四个典型的程序，并附有程序清单及注解，可供读者参考。  相似文献   

用AUTOLISP语言编程绘制三维图     

程学忠 《物探化探计算技术》2001,23(1):87-89

作者在文中介绍了在计算机辅助设计软件AUTOCAD中使用AUTOLISP语言的编程原理。用一个典型的实例，说明程序的设计过程，并附有程序清单及注解。可作为教学示例使用，很有实用价值。  相似文献   

Cloning and expression of aequorin genes from jellyfish Aequorea and characterization of aequorins activities     

Luo Wenxin  Zhang Jun  Li Shaowei  Cheng Tong  Chen Min  Li Shaojing  Xia Ningshao 《海洋学报(英文版)》2002,21(4):547-556

ＩＮＴＲＯＤＵＣＴＩＯＮＡｅｑｕｏｒｉｎｉｓａｂｉｏｌｕｍｉｎｅｓｃｅｎｔｐｒｏｔｅｉｎ ,ｉｓｏｌａｔｅｄｆｒｏｍ ｊｅｌｌｙｆｉｓｈＡｅｑｕｏｒｅａ .Ｔｈｅａｅｑｕｏｒｉｎｃｏｍｐｌｅｘｃｏｎｓｉｓｔｏｆａ 2 2 0 0 0Ｍｒａｐｏａｅｑｕｏｒｉｎｐｒｏｔｅｉｎ ,ｍｏｌｅｃｕｌａｒｏｘｙｇｅｎａｎｄｔｈｅｌｕｍｉｎｏｐｈｏｒｅｃｏｅｌｅｎｔｅｒａｚｉｎｅ .ＷｈｅｎｔｈｒｅｅＣａｉｏｎｓｂｉｎｄｔｏｔｈｉｓｃｏｍｐｌｅｘ ,ｃｏｅｌｅｎｔｅｒａｚｉｎｅｉｓｏｘｉｄｉｚｅｄｔｏｃｏｅｌｅｎ ｔｅｒａｍｉｄｅ…  相似文献   

大黄鱼(Pseudosciaena crocea)热激蛋白的基因克隆、原核表达及抗血清的制备   总被引：1，自引：1，他引：1  

张祖兴  李明云  陈炯  邬勇 《海洋与湖沼》2006,37(4):337-341

应用trizol裂解法提取象山港网箱养殖大黄鱼肝脏总RNA,采用RTPCR获得大黄鱼cDNA;通过设计特异引物进行PCR扩增,将PCR产物转化到质粒并直接测序,得到1.7kb的目的基因;同时将此目的基因连接到表达质粒pSBET中,在大肠杆菌BL21中进行诱导表达以及表达产物的分析。克隆基因的表达产物经SDSPAGE分析表明,HSP基因的蛋白分子量为60kDa左右,与阅读框的编码大小一致;表达蛋白经纯化后免疫小鼠制备抗血清。Westernblotting检测结果表明,制备的抗血清与HSP蛋白起较强的特异性反应。HSP基因的系统进化分析表明,大黄鱼与非洲爪蟾最为接近,从侧面印证了脊椎动物中两栖纲动物与鱼纲动物的亲源关系。通过大黄鱼HSP基因的提取和分析,可为以后制备出核酸探针、筛选和克隆出一批具有优良性状的基因、构建基因文库等研究工作奠定基础。  相似文献   

牙鲆dmrt1 基因的克隆及其与P450arom 基因的组织表达分析     

文爱韵  尤锋  孙鹏  徐冬冬  吴志昊  马得友  李军  张培军 《海洋科学》2010,34(11):97-102

通过基因组步移和3’RACE获得了牙鲆(Paralichthys olivaceus)dmrt1的cDNA全序列,该基因开放阅读框全长909bp,其编码的蛋白具有高度保守的DM结构域,5’UTR区含有性别相关转录因子Sox9和Sox5的结合位点。牙鲆dmrt1基因只在牙鲆的性腺中表达,且在精巢中的表达明显高于卵巢,表明牙鲆的dmrt1可能是一种性别相关基因。同时,对牙鲆的另一性别相关基因P450arom在成体各组织的表达分析结果表明,该基因除在牙鲆的性腺中有表达外,在肾脏、脾脏、鳃和脑等其他组织中也有不同程度的表达。牙鲆P450arom基因在性腺中的表达也存在两性差异,其表达模式与dmrt1的正好相反,在卵巢中的表达量明显高于精巢。  相似文献   

Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis     

LIU Fengsong  LI Fuhu  XIANG Jianhai  DONG Bo  LIU Yichen  ZHANG Xiaojun  ZHANG Liusuo 《海洋学报(英文版)》2008,27(2):81-92

A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fennero- penaeus chinensis by 3 ′and 5′ RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12. 3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus （ Litopenaeus ） setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary elec- trophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen.  相似文献   

Characterization of defensin gene from abalone <Emphasis Type="Italic">Haliotis discus hannai</Emphasis> and its deduced protein     

洪旭光  孙修勤  郑明刚  曲凌云  昝金东  张进兴 《中国海洋湖沼学报》2008,26(4):375-379

Defensin is one of preserved ancient host defensive materials formed in biological evolution. As a regulator and effector molecule, it is very important in animals＇ acquired immune system. This paper reports the defensin gene from the mixed liver and kidney cDNA library of abalone Haliotis discus hannai Ino. Sequence analysis shows that the gene sequence of full-length cDNA encodes 42 mature peptides （including six Cys）, molecular weight of 4 323 Da, and pI of 8.02. Amino acid sequence homology analysis shows that the peptides are highly similar （70% in common） to other insects defensin. Because of a typical insect-defensin structural character of mature peptide in the secondary structure, the polypeptide named Haliotis discus defensin （hd-def）, a novel of antimicrobial peptides, belongs to insects defensin subfamily. The RT-PCR result of Haliotis discus defensin shows that the gene can be expressed only in the hepatopancreas by Gram-negative and positive bacteria stimulation, which is ascribed to inducible expression. Therefore, it is revealed that the Haliotis discus defensin gene expression was related to the antibacterial infection of Haliotis discus hannai Ino.  相似文献   

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刘序俨  季颖锋  梁全强 《大地测量与地球动力学》2008,28(4):89-96

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